Working with liposomes and membrane proteins in Biacore systems
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چکیده
Solubilized proteins Detergent-solubilization of membrane proteins has proved to be a successful approach in many cases. Both ionic and nonionic detergents have been used: the choice of detergent may be crucial for retaining protein activity. Generally, detergents should be used at concentrations above their critical micelle concentration (CMC). Micelles formed above the CMC mimic a membrane environment more closely. Whether detergent needs to be included in the running buffer to prevent loss of protein activity varies from case to case.
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